# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 1 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 1.0 222.3 24.0 0.1 19.2 0.0 1.1 218.5 24.2 0.2 19.2 0.0 1.2 206.6 24.4 0.0 19.3 0.1 1.3 191.2 24.7 0.1 19.7 0.0 1.4 177.3 25.6 0.1 20.6 0.1 1.5 156.4 26.7 0.1 21.6 0.2 1.6 108.3 28.5 0.1 23.4 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 2 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 2.0 201.8 23.7 0.1 18.3 0.1 2.1 186.1 25.5 0.1 20.1 0.0 2.2 150.6 28.4 0.0 23.2 0.1 2.3 95.5 32.9 0.1 27.3 0.1 2.4 46.2 39.3 0.0 33.6 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 3 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 3.0 664.7 24.2 0.1 18.5 0.2 3.1 583.4 25.5 0.1 20.0 0.1 3.2 435.4 29.1 0.1 23.3 0.1 3.3 213.8 36.5 0.1 31.1 0.2 3.4 70.2 44.1 0.3 38.7 0.8 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 4 07072013 benzyl_viologen cell_suspension p._denitrificans no anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 4.0 1002.2 24.0 0.1 18.6 0.2 4.1 959.9 24.6 0.0 19.1 0.1 4.2 681.6 28.5 0.0 23.0 0.1 4.3 413.7 35.0 0.1 29.2 0.2 4.4 95.7 43.7 2.8 39.1 1.0 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 5 07082013 benzyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 5.0 188.9 23.4 0.1 18.6 0.1 5.1 118.8 40.0 0.3 34.4 0.3 5.2 60.6 65.3 1.3 59.3 0.6 5.3 11.3 101.9 0.0 94.8 0.0 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 6 07082013 benzyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 6.0 896.7 23.6 0.4 18.5 0.1 6.1 593.9 35.1 0.2 29.4 0.1 6.2 404.5 48.4 0.4 42.4 0.0 6.3 135.2 74.7 1.3 69.5 0.6 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 7 07082013 benzyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 7.0 853.9 28.6 0.2 23.1 0.3 7.1 682.9 35.2 0.2 29.6 0.0 7.2 393.2 46.3 0.3 40.1 0.1 7.3 238.0 66.5 0.5 59.5 0.2 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 8 07102013 methyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 8.0 194.6 23.4 0.0 18.6 0.0 8.1 99.1 40.9 0.4 34.8 0.1 8.2 50.2 60.4 0.3 53.7 0.2 8.3 11.1 100.8 nd 94.0 nd ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 9 07102013 methyl_viologen cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 9.0 812.6 23.9 0.0 18.5 0.0 9.1 655.9 32.1 0.1 26.1 0.0 9.2 373.4 49.2 0.2 41.9 0.1 9.3 59.8 100.3 0.0 89.0 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 10 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 10.0 915.4 24.7 0.0 18.7 0.0 10.1 880.9 29.5 0.0 23.7 0.0 10.2 736.8 35.3 0.0 29.6 0.1 10.3 407.3 54.9 0.1 48.3 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 11 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 11.0 873.2 27.8 0.0 21.8 0.0 11.1 772.5 30.7 0.1 24.3 0.0 11.2 548.4 39.7 0.2 33.1 0.1 11.3 507.8 45.4 0.0 38.4 0.1 11.4 310.5 59.9 0.1 52.0 0.0 11.5 181.3 72.2 0.1 63.0 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 12 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 12.0 904.2 24.2 0.0 18.5 0.1 12.1 780.3 28.8 0.0 23.2 0.0 12.2 705.1 32.5 0.1 26.9 0.1 12.3 618.4 37.5 0.4 31.7 0.0 12.4 431.4 49.6 0.1 43.1 0.1 12.5 313.6 57.4 0.2 50.4 0.2 12.6 234.0 66.3 0.3 58.9 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 13 11082014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 13.0 888.6 24.3 0.1 18.7 0.2 13.1 847.5 27.4 0.0 21.9 0.0 13.2 757.9 30.8 0.1 24.8 0.0 13.3 669.6 34.2 0.2 28.0 0.1 13.4 546.4 40.9 0.0 34.7 0.1 13.5 464.3 46.0 0.1 39.4 0.0 13.6 385.9 51.9 0.1 45.2 0.1 13.7 234.2 66.8 0.2 59.3 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 14 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 14.0 246.1 24.1 0.3 19.2 0.0 14.1 142.4 35.4 0.3 30.3 0.3 14.2 87.8 45.7 5.2 40.9 5.3 14.3 49.4 61.1 0.1 58.0 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 15 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 15.0 197.7 24.0 0.1 18.8 0.1 15.1 141.3 33.9 0.1 28.4 0.0 15.2 101.1 43.5 0.3 39.4 0.2 15.3 66.0 52.2 0.2 47.3 0.2 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 16 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 16.0 994.3 26.0 0.1 20.6 0.0 16.1 816.9 29.4 0.1 23.9 0.1 16.2 779.6 32.3 0.1 26.7 0.1 16.3 685.5 36.4 0.0 30.7 0.0 16.4 600.1 39.4 0.2 33.8 0.0 16.5 531.1 41.9 0.0 37.0 0.3 16.6 445.7 47.2 0.0 41.6 0.0 16.7 427.1 48.8 0.1 44.6 0.3 16.8 303.3 59.1 0.3 53.0 0.1 16.9 217.9 68.9 0.1 61.6 0.2 16.1 129.2 80.3 0.2 72.8 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 17 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 17.0 878.2 26.0 0.0 20.7 0.1 17.1 779.6 31.2 2.2 23.8 0.4 17.2 676.7 34.0 0.0 28.3 0.1 17.3 574.9 38.3 0.2 32.4 0.1 17.4 463.2 44.5 0.0 38.2 0.1 17.5 357.0 52.1 0.0 45.4 0.2 17.6 298.9 59.5 0.0 52.7 0.1 17.7 224.5 65.2 0.0 58.3 0.0 17.8 162.1 72.8 0.0 65.4 0.2 17.9 117.2 78.2 0.0 71.5 0.2 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 18 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 18.0 834.4 24.9 0.2 19.6 0.1 18.1 789.5 26.8 0.0 21.4 0.1 18.2 757.7 28.6 0.1 23.2 0.1 18.3 720.5 30.9 0.0 25.5 0.2 18.4 665.8 33.4 0.1 27.9 0.0 18.5 563.9 37.2 0.0 31.5 0.0 18.6 487.3 41.0 0.0 35.3 0.0 18.7 431.4 44.5 0.1 38.4 0.0 18.8 422.7 47.2 0.2 41.0 0.0 18.9 330.7 53.4 0.0 47.0 0.1 18.1 283.6 58.7 0.0 52.2 0.1 18.11 214.6 65.1 0.1 58.1 0.3 18.12 182.9 70.4 0.0 63.4 0.1 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 19 03202014 hydroquinone cell_suspension p._denitrificans yes anaerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 19.0 985.5 25.2 0.0 19.9 0.0 19.1 842.1 28.1 0.1 22.8 0.2 19.2 765.4 31.6 0.1 25.9 0.2 19.3 671.2 35.0 0.0 29.5 0.1 19.4 570.5 39.2 0.1 33.3 0.2 19.5 514.7 42.5 0.0 36.4 0.2 19.6 441.3 45.8 0.1 39.6 0.2 19.7 420.5 48.6 0.0 42.4 0.1 19.8 319.7 56.7 0.2 49.6 0.2 19.9 283.6 62.5 0.1 55.6 0.2 19.1 233.2 64.0 0.1 59.5 0.0 19.11 210.2 67.6 0.1 61.1 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 20 06182014 methyl_viologen cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 20.0 879.3 26.7 0.0 21.8 0.1 20.1 747.9 31.7 0.1 26.5 0.2 20.1 572.7 39.6 0.0 34.0 0.2 20.1 397.5 50.5 0.1 44.5 0.2 20.1 237.6 64.1 0.5 57.8 0.4 20.1 96.4 75.7 0.7 69.9 0.6 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 21 06182014 hydroquinone cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 21.0 970.2 24.5 0.0 19.2 0.3 21.1 945.0 25.2 0.0 19.9 0.6 21.2 844.2 27.7 0.0 21.4 1.6 21.3 869.4 28.3 0.0 22.8 0.5 21.4 811.4 29.4 0.1 23.7 0.8 21.5 781.8 30.2 0.0 24.7 0.9 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 22 06182014 hydroquinone cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 22.0 961.4 25.3 0.0 20.4 0.1 22.1 854.1 28.3 0.1 23.4 0.1 22.2 753.4 31.7 0.0 26.8 0.1 22.3 638.4 36.5 0.2 31.5 0.1 22.4 537.6 41.4 0.0 36.2 0.1 22.5 440.2 46.4 0.2 41.4 0.0 22.6 330.7 51.8 0.3 47.3 0.3 22.7 213.5 65.6 0.2 59.6 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 23 06182014 hydroquinone cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 23.0 935.1 25.7 0.1 20.3 0.0 23.1 816.9 29.2 0.1 24.1 0.1 23.2 648.2 34.4 0.0 29.1 0.1 23.3 579.3 38.1 0.0 33.4 0.1 23.4 442.4 46.4 0.0 40.6 0.0 23.5 325.2 54.2 0.4 48.2 0.0 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 24 07102014 methyl_viologen cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 24.0 896.8 26.2 0.2 20.9 0.1 24.1 894.6 27.7 0.3 22.4 0.1 24.2 860.7 29.6 0.3 24.3 0.2 24.3 721.6 32.3 0.0 27.3 0.0 24.4 636.2 35.8 0.0 30.5 0.0 24.5 563.9 39.3 0.1 34.3 0.0 24.6 523.4 41.7 0.0 37.3 0.0 24.7 286.9 57.3 1.4 51.3 0.5 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 25 07102014 methyl_viologen cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 25.0 893.5 26.2 0.5 21.1 0.3 25.1 832.2 27.6 0.0 22.8 0.1 25.2 815.8 29.3 0.0 24.4 0.1 25.3 730.4 32.2 0.1 27.3 0.3 25.4 646.1 34.7 0.0 29.9 0.1 25.5 622.0 37.5 0.0 33.0 0.0 25.6 572.7 40.4 0.0 35.9 0.0 25.7 443.5 45.6 0.1 40.2 0.1 25.8 387.6 49.8 0.2 44.5 0.2 25.9 321.9 55.0 0.2 49.3 0.1 26.1 283.6 58.8 0.1 53.3 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 26 07102014 hydroquinone cell_suspension p._denitrificans yes aerobic 20 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 26.0 998.6 24.9 0.1 19.7 0.3 26.1 919.8 26.8 0.1 21.7 0.1 26.2 866.1 28.8 0.0 23.7 0.1 26.3 866.1 30.3 0.1 25.3 0.1 26.4 791.7 32.0 0.2 26.9 0.2 26.5 740.2 34.1 0.0 29.3 0.2 26.6 743.5 33.2 0.1 28.0 0.2 26.7 681.1 36.5 0.1 31.3 0.0 26.8 582.5 42.2 0.0 37.0 0.0 26.9 555.2 43.1 0.3 37.9 0.1